Freddy Dardenne

History

Member for

1 year 29 weeks

Professional information

Position in the lab

Research Manager

Curriculum Vitae

At present I’m enjoying being a research manager at the University of Antwerp in the field of ecotoxicolgy and ecophysiology. Our group has a lot to offer concerning effect and impact studies of environmental stressors on a range of biota. Most important we can help in understanding why which effect / impact occurs as a response to a stressor, e.g. a pollutant or environmental change. The tools we use are traditional ecotoxicological tests, supported and extended by several molecular techniques (e.g. transcriptomics, proteomics, metabolomics) giving physiological and mechanistic information to solve complex cause-effect relationships. We deliver acute and chronic toxicity endpoints and more complex mode of action studies on pure compounds, mixtures, environmental samples and acknowledge our findings through field studies and comparisons.
My research expertise goes back to 1986 and covers a broad range of life sciences. I picked up my molecular skills in agricultural crop improvement (DNA, RNA, protein), my diagnostic knowhow as a head of a production facility of human diagnostics (mainly ELISA systems) and a thorough process microbiology knowledge in several years of industrial contract research (Süd Zucker, Cargill, New Holland, …). Besides a broad knowhow on microbiology, especially bacterial population dynamics and microbial traceability are the main skills to mention. The knowhow on ecotoxicology and more specifically the use of in vitro testing, I picked up during my PhD at the University of Antwerp.
An important red line, next to the scientifical and technological emphasis in my professional career, is the importance of quality management systems and alongside the financial analysis of the operational and quality aspects of research and/or routine labs

Online bibliography

http://anet.ua.ac.be/acadbib/ua/01797

Selected publications

1.Robbens Johan, Dardenne Freddy, Devriese Lisa, de Coen Wim, Blust Ronny. Escherichia coli as a bioreporter in ecotoxicology;
Applied microbiology and biotechnology - ISSN 0175-7598 - 88:5(2010), p. 1007-1025
http://dx.doi.org/doi:10.1007/s00253-010-2826-6

2. Nobels I, Dardenne F, De Coen W, Blust R. 2010. Application of a multiple endpoint bacterial reporter assay to evaluate toxicological relevant endpoints of perfluorinated compounds with different functional groups and varying chain lenth. Toxicology in Vitro, 24 (2010) 1768–1774.

3. Dardenne F, Nobels I, De Coen W, Blust R. 2008. Mixture toxicity and gene inductions: can we predict the outcome? Environmental Toxicology and Chemistry 27:509-518.

4. Dardenne F, Van Dongen S, Nobels I, Smolders R, De Coen W, Blust R. 2008. Mode of action clustering of chemicals and environmental samples on the bases of bacterial stress gene inductions. Toxicological Sciences 101:206-214.

5. Dardenne F, Nobels I, De Coen W, Blust R. 2007. Dose-response relationships and statistical performance of a battery of bacterial gene profiling assays. Applied Microbiology and Biotechnology: Environmental Biotechnology 75:223-234.

6. Dardenne F, Smolders R, De Coen W, Blust R. 2007. Prokaryotic gene profiling assays to detect sediment toxicity: evaluating the ecotoxicological relevance of a cell-based assay. Environmental Science & Technology 41:1790-1796.

7. Eisentraeger A, Reifferscheid G, Dardenne F, Blust R, Schofer A. 2007. Hazard vharacterization and identification of a former ammunition site using microarrays, bioassays, and chemical analysis. Environmental Toxicology and Chemistry 26:634-646.

8. Dardenne F, Seurinck J, Lambert B, Peferoen M. 1990. Nucleotide sequence and deduced amino acid sequence of a cryIA(c) gene variant from Bacillus thuringiensis. Nucleic Acids Research 18.

Selected presentations

1. Leon Paumen M, De Jong I, Groen R, Van der Meulen M, Dardenne F, Covaci A, Bervoets L, Blust R, Comber M, De Schutter D, Parkerton T, Kraak M. 2007. Triad assessment of a Belgium lowland stream receiving effluent of a polymer production facility. Abstracts of 17th annual meeting of SETAC-Europe, Porto, Portugal. 20-24 May p 193-194.

2. Nobels I, Vanparys C, Dardenne F, Van den Heuvel R, Vercauteren J, Wauters E, Witters H, De Coen W. 2007. In vitro evaluation of air quality: a HepG2 based reporter assay. Abstracts of 17th annual meeting of SETAC-Europe, Porto, Portugal. 20-24 May p 220.

3. Nobels I, Dardenne F, Pussemier L, De Coen W, Blust R. 2007. Reporter gene expression analysis for screening of food contaminants. Abstract Toxicology Letters 172S: S198.

4. Dardenne F, Nobels I, De Coen W, Blust R. 2006. Bacterial class I bioreporters for ecotoxicological assessments. Abstracts of the 24th annual meeting of ESCPB Antwerp, Belgium. 17-20 September p 56.

5. Dardenne F, Nobels I, Blust R, De Coen W. 2006. Statistical considerations in the screening of environmental samples using a battery of gene expression assays. Abstracts of 16th annual meeting of SETAC-Europe, Den Haag, The Netherlands. 7 – 11 May p 280.

6. Nobels I, Dardenne F, De Coen W, Blust R. 2006. Detection and discrimination between different commercial formulations of glyphosate by means of gene profiles. Abstracts of the 24th annual meeting of ESCPB Antwerp, Belgium. 17-20 September p 83.

7. Nobels I, Dardenne F, De Coen W, Blust R. 2006. Reporter gene expression analysis for rapid screening of (re)emerging risks. Abstracts of the 24th annual meeting of ESCPB Antwerp, Belgium. 17-20 September p 84.

8. Nobels I, Dardenne F, Maras M, De Coen W, Blust R. 2006. Use of gene expression profiles for detection of anthropogenic and naturally occurring microcontaminants in food. Abstracts of 16th annual meeting of SETAC-Europe, Den Haag, The Netherlands. 7 – 11 May p 198.

9. Nobels I, Maras M, Dardenne F, Van de Vijver KI, Blust R, De Coen W. 2006. Mode of action studies of 19 perfluorinated compounds (PFCs) using gene expression profiles. Abstracts of 16th annual meeting of SETAC-Europe, Den Haag, The Netherlands. 7 – 11 May p 296.

10. Dardenne F, Smolders R, Naudts B, Cocker D, De Coen W, Blust R. 2005. In vitro gene profiling assays for the detection of microcontaminants in environmental and food matrices. SETAC Europe 15th Annual Meeting Lille, France. 22-26 May p 287.

11. Nobels I, Dardenne F, Hoff, P, Van de Vijver K, Blust R, De Coen W. 2005. Use of gene profiling assays in toxicity studies with perfluoroalkyl compounds. SETAC Europe 15th Annual Meeting Lille, France. 22-26 May p 286.

12. Blust R, Dardenne F, Naudts B, Cocker D, De Coen W. 2004. Routine gene expression profiling of environmental samples: adding biological realism to chemical monitoring. SETAC 4th World Congress, Portland, Oregon, USA. 14-18 November p 465.

13. Dardenne F, Smolders R, Naudts B, Smet A, De Coen W, Blust R. 2004. Prokaryotic and eukaryotic gene profiling assays: the ecotoxicological relevance of in vitro results? SETAC 4th World Congress, Portland, Oregon, USA. 14-18 November p 49.

14. Dardenne F, Willems KA, De Coen W, Blust R. 2004. Prokaryotic gene profiling assays as a novel tool to assess the mechanisms of mixture toxicity. SETAC Europe 14th Annual Meeting Prague, Czech Republic. 18-22 April p 6-7.

15. De Coen W, Dardenne F, Naudts B, Cocker D, Blust R. 2004. Alternative testing methods and their implementation in REACH: the power of gene expression profiling. SETAC 4th World Congress, Portland, Oregon, USA. 14-18 November p 186.

16. Dardenne F, Potters E, Smolders R, Huyskens G, De Coen W, Blust R. 2003. Assessment of the Ecotoxicological Quality of Surface Waters through Stress Gene Profiling, using Bio-mimetic Solid Phase Extraction. SETAC 24th Annual Meeting Austin, Texas. 9-13 November p 104.

17. Van de Vijver K, Hoff P, Dardenne F, Van Dongen W, Esmans E, Blust R, De Coen W. 2003. Distribution and effects of perfluorooctane sulfonic acid (PFOS) in aquatic organisms. VLIZ Young Scientists' Day Brugge, Belgium. 28 February VLIZ Special Publication, 12:51.

18. Brack W, Meissner B, De Coen W, Dardenne F, Blust R, Altenburger R, Küster E, Wenzel KD, Schüürmann G. 2002. Sediment Quality Criteria for Photomodified PAHs. SedNet Conference Venice, Italy, 22-23 April p?.

19. Dardenne F, Vlaeminck A, Willems K, De Coen W, Blust R. 2002. Evaluation of a battery of prokaryotic stress gene assays for environmental toxicity screening purposes. SETAC Europe 12th Annual Meeting Vienna, Austria. 12–16 May p 40.

20. Dardenne F, Willems KA, Claes P, De Coen W, Blust R. 2002. Analysis of the toxicity of simple mixtures through gene profiling using transgenic human cell lines. SETAC 23rd Annual Meeting in North America, Salt Lake City, Utah, USA. 16-20 November p 9.

21. De Coen W, Moens L, Van der Ven K, Hoff P, Caturla-Goñi M, Vlaeminck A, Dardenne F, Willems K, Blust R. 2002. Assessment of toxicological modes of action of chemicals: from conventional biomarkers to toxicogenomics. SETAC Europe 12th Annual Meeting Vienna, Austria. 12–16 May p 19.