Danio rerio

Abstract

This study investigates the importance of water and food as exposure sources of three model contaminants with different physico-chemical properties and modes of action to zebrafish. It is explored to what extend uptake via water or food results in different responses and toxic effects. The responses and effects are studied at molecular, cellular and organismal level using genomics, proteomics and physiological approaches. The project aims to provide a in depth understanding of how different substances interact with a model system taking into account key factors such as developmental stage, exposure route and exposure time. The results of the genomics and proteomics analysis should considerable enlarge our understanding of the molecular mechanisms of toxicity and defense.

Perfluorinated organic compounds (PFOCs) are manufactured for over 50 years in enormous quantities and are used in materials such as wetting agents, foam fire extinguishers and stain resistant treatments for leather, paper and clothing. Due to their environmental persistence and tendency to bioaccumulate, the global concern for the potential toxicity of these organic compounds has strongly been rising during the last decade.